A critical assessment of the Flory-Huggins (FH) theory to predict aqueous two-phase behaviour

This article provides an analysis on published models used to calculate phase separation in aqueous two-phase systems (ATPS) based Flory-Huggins (FH) theory, terms of problem formulation and mathematical solving algorithm. An integrated algorithm is presented, showing different approaches using the FH theory. The involves estimation interchange energy, calculation compositions. Based experimental data, thermodynamic model can provide a useful framework perform sensitivity parameters, order understand influence salt type, polymer molecular mass, ionic strength separation. However, this model, restricted entropic enthalpic terms, cannot quantitatively describe data. occurs mainly because strong random errors energy not being exact description ATPS. After providing literature overview analysis, we bring field application theory for selecting ATPS its limitations.